BioProfile® Basic, 100, 300, 400, pHOx

Nova Bioprofile Series

BioProfile Automated Analyzers for Mammalian Cell Culture

  • Seven models and test menus
  • All results available in 3 minutes or less
  • Automatic one-button operation
  • 40-position batch processing capability
  • Automatic analyzer calibration
  • No external sample dilutions
  • No gas tanks required

Nova BioProfile analyzers include four models of fully automated cell culture analyzers with test menus of two to ten tests, including chemistry, gases, and electrolytes. Each model offers automatic calibration, one-button operation, and 40 position batch processing capability.

Model Nutrients/Metabolites
BioProfile Basic 2 Glucose, Lactate
BioProfile Basic 4 Glutamine, Glutamate, Glucose, Lactate
Model Nutrients/Metabolites Acid/Base Status Electrolytes Calculated Parameter
BioProfile 100 Plus Glutamine, Glutamate, Glucose, Lactate, Ammonium pH Sodium, Potassium Osmolality
BioProfile 400 Glutamine, Glutamate, Glucose, Lactate, Ammonium pH, PO2, PCO2 Sodium, Potassium Osmolality, Air Saturation, CO2, Saturation, HCO3

BioProfile Analyzers for Bacterial Fermentation

BioProfile 300A and 300B offer glucose. lactate, acetate, ammonium, pH, sodium, potassium and a choice of either phosphate or glycerol. These systems offer measurement ranges specifically suited to bacterial fermentations. Both models feature automatic calibration, one-button analysis and batch processing capability

Model Nutrients/Metabolites Acid/Base Status Electrolytes Calculated Parameter
BioProfile 300 A Glucose, Lactate, Ammonium, Acetate, Phosphate* pH Sodium, Potassium Osmolality
BioProfile 300 B Glucose, Lactate, Ammonium, Acetate, Glycerol pH Sodium, Potassium Osmolality

* Also reports phosphorus

BioProfile pHOx

BioProfile pHOx is designed to measure acid/base and respiratory parameters from mammalian cell culture and bacterial fermentations. This compact, easy to use analyzer provides rapid, accurate results throughout a wide range of pH, PCO2, and PO2 levels encountered in cell cultures and fermentations.

Model pH/Gases Calculated Parameters
BioProfile pHOx pH, PO2, PCO2 CO2 Saturation, Air Saturation, HCO3, Total CO2

BioProfile Applications

Developed in conjunction with leading biotechnology companies, BioProfile Analyzers provide those chemistry assays necessary to address the testing requirements of small to large scale bioprocessing, encompassing:

  • Research and Development
  • Process Development
  • Pilot Plant
  • Production

Applications of the BioProfile include:

  • Monitoring the progress of bioreactor runs
  • Determining the consumption and production of key metabolites
  • Identification of growth-limiting nutrients
  • Design of feeding strategies
  • Calibration of bioreactor probes
  • Measurement of cellular respiration
  • Balancing media electrolytes
  • Control levels of waste product

Time/Labor Savings

BioProfile’s comprehensive test menus allow consolidation of testing into a single analyzer. This results in significant time and labor savings, as well as real-time process control capabilities.

BioProfile’s fast analysis time and consolidated testing offer the following advantages:

  • Eliminate the requirement for taking multiple samples and large sample volumes from reactor vessels
  • Provide real-time access to test results, enabling real-time process control
  • Substantially decrease labor, capital equipment, and reagent costs
  • Improve the ability to effectively monitor and control bioreactor processes
  • Improve process reliability and reproducibility
  • Speed optimization of feeding strategies
  • Improve manufacturing yield and quality
bioprofile analysis time

Measuring Technologies


Potentiometric Electrodes (pH, PCO2, NH4+, Na+, K+, Acetate)
Potentiometric-based electrodes measure charged ions and have a sensing membrane that is selective to the ion being measured. They develop a voltage proportional to the concentration of the measured ion.

Amperometric Electrodes (PO2, Glucose, Lactate, Glutamine, Glutamate)
The basic amperometric electrode is the oxygen electrode, which consists of an oxygen-permeable membrane covering a platinum cathode.

Glucose, lactate, glutamine, and glutamate biosensors are amperometric electrodes that have immobilized enzymes in their membranes. They develop a current proportional to the substrate being measured.

Glucose Electrode

Photometry (Phosphate and Glycerol)
The photometry method measures the change in light transmission through a sample mixture to calculate the analyte concentration in the sample.

The phosphate assay is based on the reaction of phosphate ions with ammonium molybdate to form an ammonium phosphomolybdate complex which is converted to a blue color using a reducing agent.

The glycerol assay is based on oxidation of glycerol into formaldehyde using sodium meta-periodate in buffered solution.

Analytical Performance

Unlike in-situ monitoring devices, BioProfile Analyzer’s automated calibration protocol provides precise quality control and maintains consistency of test results. Further, BioProfile’s automated measurement provides faster, more accurate results than off-line methods.

Results obtained from the BioProfile Analyzer show excellent correlation with conventional techniques.

Correlation Data

Correlation data Correlation graphs

Automated Testing

Fully-Automated Testing

BioProfile Analyzers are fully automated and designed for easy use by any operator. After the “ANALYZE” key is pressed, the sample is automatically aspirated, and the analysis is performed without further operator intervention. An on-board computer monitors the analysis cycle from sample aspiration to results presentation. The operational status of the BioProfile Analyzer is continuously displayed; any deviation from normal operation is promptly reported to the operator with any suspect data flagged.

Analyze button

Presentation of Test Results

BioProfile Analyzers report test results on a CRT or monochrome display and a built-in printer. The analyzer can be programmed to alert the operator automatically when test data are outside pre-determined normal ranges and critical limits. Out-of-range test samples are flagged on all data print-outs.

All results can be stored for review individually or for trend analysis, using the optional BioProfile Data Management System (BDM), or via an external computer with appropriate data communications software.

BioProfile 400 test results chart

Batch Processing

Automated Batch Processing

BioProfile Basic, 100 Plus, 300 and 400 offer a 40-position sample tray that enables batch processing with full walkaway automation. The sample tray will accommodate a variety of tube sizes including:

  • 500 µL – 2 mL Eppendorf tubes
  • 500 µL – 4 mL sample cups
  • 5 mL – 7 mL test tubes

A complete 40-sample tray can be analyzed in approximately 80 minutes.

BioProfile 100 tray

Mobile Carts

Mobile workstation carts with UPS power supplies are available for all BioProfile models. These carts allow transport of the analyzer to any location.

Mobile workstation carts with UPS power supplies

Measurement Ranges

BioProfile measurement ranges

OPC Server

Nova’s OPC Server software allows the BioProfile Analyzer to transfer analytical data on key chemistries to any bioreactor controlling software that is OPC compliant. This critical data allows the controller to initiate real time feedback control to optimize the bioprocess. The OPC Server software can be installed on the Nova Biomedical Data Manager (BDM) or on an ancillary computer.

OPC is a published industrial standard for system interconnectivity that provides a standards-based infrastructure for the exchange of process control data. It uses Microsoft COM and DCOM technology to enable applications to exchange data on one or more computers using client server architecture.

OPC defines a common set of interfaces so that each application retrieves data in exactly the same format, regardless of the data source. Using OPC standards, data can be transmitted from any data source to any OPC-compliant application including HMI/SCADA, data historians, spreadsheets, and databases. The result is a complete, out-of-the-box, plug and play communication solution.

True interoperability reduces implementation costs and builds a fully scaleable system to accommodate your current and future bioprocessing requirements.

Typical system

21 CFR Part 11 Compliance

21 CFR Part 11 describes regulatory requirements governing electronic signatures and records that assure the integrity of data from an analytical instrument such as the BioProfile Analyzer.

The BioProfile Data Management System (BDM) incorporates an effective data archive and restoration system as well as adequate security features and audit trails to automatically monitor the creation, modification, and deletion of electronic records. These features create full compliance with 21 CFR Part 11 requirements.


The BioProfile Instrumentation Qualification (IQ) protocol provides comprehensive information to assist in validating the BioProfile Analyzer for use in your bioprocess.

The IQ protocol includes:

  • System documentation
  • Pre-installation checks
  • Installation procedure checks
  • Installation performance qualification
  • Instrument/system control verification

The BioProfile Instrumentation Qualification/Operation Qualification (IQ/OQ) service includes all Installation Qualification procedures outlined above, plus:

  • Performance of IQ/OQ procedures
  • All required NIST traceable standard materials
  • Statistical analysis and final report

These optional services can be performed either during installation or at a later date.

BioProfile® On-Line Autosampler

BioProfile On-lin Autosampler

Automated Sampling

The BioProfile On-Line Autosampler connects up to four bioreactors or fermentation vessels to a BioProfile Basic, 100 Plus, 300, or 400 analyzer for direct, automated sampling. Sampling frequency is programmed into the BioProfile Data Management System (BDM) by the user for data storage and/or review and trend analysis. The BDM is included with the On-Line Autosampler.

Oxygen graph

Feedback Control System

A Feedback Control System, available as an option to the On-Line Autosampler, allows automated introduction of nutrients when preset limits are reached. Addition of nutrients is signaled by the BioProfile Data Management System (BDM) when a specified parameter measures outside the programmed range. As many as eight feedback loops can be configured.

Certifications & Compliance: ISO 13485:2003 Quality System Registration, IVDD, Meets IEC 61010, UL, CSA Standards
FDA Labeling: For in-vitro diagnostic use.
Patented Technology. Patents 6,287,451 / 6,837,976 / EP 1 212 609 / CA 2,375,092 / EP 1497 449 / JP 405 0078
Specifications subject to change without notice.