BioProfile® FLEX


Nova Bioprofile Flex

BioProfile FLEX Analyzer


One Automated Analyzer for Fast, Comprehensive Cell Culture Analysis

BioProfile is a chemistry/cell viability modular instrument that measures up to 15 key cell culture attributes. By combining as many as five separate instruments into one easy-to-use instrument, BioProfile FLEX simplifies workflow and saves time, labor, and operating cost versus multiple instruments. Applications of BioProfile FLEX include:

  • Monitoring the progress of bioreactor runs
  • Determining consumption and production of key metabolites
  • Identification of growth-limiting nutrients
  • Design of feeding strategies
  • Calibration of bioreactor probes
  • Measurement of cellular respiration
  • Balancing media electrolytes
  • Control levels of waste product.

Modular, Field Upgradeable System

The modular design of BioProfile FLEX can be customized with one to four analytical modules to consolidate up to 15 vital cell culture tests. modular field upgradable analysis system

One Fast Analysis

Saves as much as 30 minutes per sample compared to using multiple instruments; analysis as fast as 2 minutes per sample.

One Integrated Data Report

Simplifies data collection, analysis, archiving, and regulatory compliance.

One Intuitive User Interface

Saves 30 or more operator steps compared to multiple instrument interfaces

One Small, 1 mL Sample

Conserves cell culture mass and end product.

One Consolidated Workstation

Saves up to 15-20 square feet of valuable bench space and saves hours of maintenance each month compared to multiple instruments.


Patented Technology. Patents 6,287,451 / 6,837,976 / EP 1 212 609 / CA 2,375,092 / EP 1497 449 / JP 405 0078

Specifications subject to change without notice.

Optional Equipment

On-Line Autosampling

  • An optional On-Line Autosampler connects BioProfile FLEX to as many as ten bioreactors. Scheduling of sampling
    into the BioProfile FLEX analyzer is user programmable.
  • Individual syringe or cup samples can also be analyzed during periods when on-line autosampling is not scheduled.

Bioprofile Flex with On-Line Autosampler

Sample Retain Collection System

The BioProfile FLEX Sample Retain Collection System is an accessory used to automatically collect and store bioreator samples in a refrigerated environment for further analysis. The addition of the Sample Retain Collection System allows user-selectable retained sample volumes from 1.0 to 50 mL and selectable storage temperature down to 3˚C. Sampling and storage can be initiated manually or on an automated schedule.

Advanced OPC Connectivity

The Nova OPC Connectivity Suite integrates BioProfile FLEX with any OPC compliant devices such as bioreactor controllers, data historians, laboratory information management systems (LIMS), and plant management systems.

    Nova’s OPC Connectivity Suite features:

  • Automated Bi-directional Flow of Data and Control Commands
  • Data Archiving (DA) and Historical Data Archiving (HDA) Capability
  • Easy “handshake” connection to any OPC compliant device
  • Connectivity verification
  • BioReactor Feedback Control
  • Remote monitoring of BioProfile FLEX status and data

Certifications & Compliance: ISO 13485:2003 Quality System Registration, IVDD, Meets IEC 61010, UL, CSA Standards FDA Labeling: For in-vitro diagnostic use.

Patented Technology. Patents 6,287,451 / 6,837,976 / EP 1 212 609 / CA 2,375,092 / EP 1497 449 / JP 405 0078

Specifications subject to change without notice.

Specifications

bioprofile flex Sample Analysis Time: 2.5 minutes (Gases only – PO2 + PCO2)

4.0 minutes (Chemistry/Gas & Osmolality)

6.25 minutes (All 16 tests) Operating Temperature Range: 15-30°C (59-86°F) Operating Relative Humidity Range: 20-85% Sample Size: 1 mL (for all modules) Sampling Options: Individual via syringe/cup

Automated batch using 20-position sampling tray

On-line, automated from up to ten bioreactors Electrical Requirements: 90-264 VAC, 50 to 60 Hz (Universal Power Supply) System Size: Height: 20 in (50 cm)

Width: 26 in (66 cm)

Depth: 20 in (50 cm) System Weight: Analyzer: 94 lbs (42.6 kg) without reagents

Host Computer Monitor/CPU: 41.5 lbs (18.8 kg) Safety Certifications: TUV: IEC 61010-1:2001 Quality Systems Certifications: TUV: ISO9001:2000 FDA Registered, OPC Compliant, PAT Compatible, 21 CFR Part 11 Compliant

Chemistry/Gas Module

Assay Measurement Range Resolution Method
Glucose 0.2–30.0 g/L* 0.01g/L Biosensor
Lactate 0.2–10.0 g/L* 0.01g/L Biosensor
Glutamine 0.2–12.0 mmol/L* 0.01 mmol/L Biosensor
Glutamate 0.2–12.0 mmol/L* 0.01 mmol/L Biosensor
Ammonium 0.2–25.0 mmol/L 0.01 mmol/L Direct ISE
pH 5.000–8.000 0.001 Direct ISE
PCO2 3.0–300.0 mmHg 0.1 mmHg Direct ISE
PO2 3.0–800.0 mmHg 0.1 mmHg Clarke Electrode
Sodium 40–300 mmol/L 0.1 mmol/L Direct ISE
Potassium 1.0–100.0 mmol/L 0.01 mmol/L Direct ISE
Calcium 0.10–10.0 mmol/L 0.01 mmol/L Direct ISE

Calculated Tests:O2 Saturation; CO2 Saturation; Temp. Corrected pH, PCO2, PO2 *Ranges Reflect User Selectable 1:2 Dilution

Osmolality Module

Assay Measurement Range Resolution Method
Osmolality 0–1500 mOsm/kg 1 mOsm/kg Freezing Point

Cell Density/Viability Module

Assay Measurement Range Resolution Method
Cell Diameter 8 – 50 μm N/A Digital Imaging
Detectable Density 50,000 – 100,000,000cells/mL** N/A Digital Imaging
Viability 0 – 100% N/A Digital Imaging

Phosphate Module

Measurement Range 0.20-10 mmol/L
Sample Size 500 µL

**Ranges Reflect User Selectable 1:2, 1:4, 1:5, 1:10 Dilution

IgG Module

Measurement Range: 0.10–10.00 g/L* *Ranges Reflect User Selectable 1:2 Dilution


Patented Technology. Patents 6,287,451 / 6,837,976 / EP 1 212 609 / CA 2,375,092 / EP 1497 449 / JP 405 0078

Specifications subject to change without notice.

Level 1 Expiration
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